Culture medium, cell culture kit and cell culture processes

Culture medium, cell culture kit and cell culture processes

  • CN 102,787,093 B
  • Filed: 05/17/2011
  • Issued: 11/07/2017
  • Est. Priority Date: 05/17/2011
  • Status: Active Grant
First Claim
Patent Images

1. a kind of purposes of kit in culture epithelial cell, the kit includes culture medium, calcium component, serum and ROCKInhibitor, wherein the culture medium includes conditioned medium, the conditioned medium includes feeder cells secretion and/or extractionThing, the epithelial cell is non-Eponychium cell, the non-keratinocyte epithelial cell be oral cavity, tracheae, bronchial, lungEpithelium, gastric epithelial, colon, liver cell, pancreas, bladder, ovarian epithelium, thyroid, prostate, mammary gland, it is tonsilar, the epithelial cell is primary cell.The kit is used to prepare in the culture medium for including serum, calcium component and ROCK inhibitor, the culture medium, calcium componentContent is calculated as 0.1 μ

  • M~10mM with calcium ion, and the content of serum is 2.0~20v/v%, and the content of ROCK inhibitor is 0.1 μ

    M~1mM.

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