A kind of detection method of COL4A5 transgenation

A kind of detection method of COL4A5 transgenation

  • CN 102,787,167 B
  • Filed: 08/15/2012
  • Issued: 03/02/2016
  • Est. Priority Date: 08/15/2012
  • Status: Active Grant
First Claim
Patent Images

1. , for a detection method for the COL4A5 transgenation of non-treatment and non-diagnostic object, comprise the steps:

  • A) skin histology departing from human body is placed in conserving liquid and carries out pre-treatment;

    B) extracting directly RNA from the skin histology of described pretreated disengaging human body;

    C) RT-PCR is carried out to the RNA of described extraction, obtain pcr amplification product;

    D) the described pcr amplification product obtained is checked order, sequencing result and normal human COL4A5 gene order are compared, analyze whether producer suddenlys change;

    Wherein said method also comprises;

    before extraction RNA, be stored in by the skin histology of described disengaging human body and preserve in solution, the temperature of storage is 4-37 DEG C, storage time≤

    7 days;

    Wherein, described steps A) comprising;

    A1) carry out milled processed after being mixed with liquid nitrogen by the skin histology departing from human body, obtain the grinding mixture containing skin histology;

    A2) extracting RNA to containing adding Trizol reagent in the grinding mixture of skin histology, obtaining RNA extracting solution;

    A3) purifying RNA extracting solution, obtains RNA;

    Wherein, the surface-area of described skin histology is 4 to 8mm 2.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×