A kind of High water retention microbe medium and preparation method thereof

A kind of High water retention microbe medium and preparation method thereof

  • CN 102,796,686 B
  • Filed: 08/14/2012
  • Issued: 10/20/2017
  • Est. Priority Date: 08/14/2012
  • Status: Active Grant
First Claim
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1. a kind of preparation method of the microbiological culture media with high-moisture-retention, it is characterised in that it the step of it is as follows:

  • 1)

    The preparation of super absorbent resin(

    1)

    To be 1 with monomer weight ratio first in ice-water bath:

    10~1;

    1 alkali is dissolved in water, or is in principal monomerAlkali is added without during non-acidic monomers to be directly dissolved in water;

    Then it is slowly dropped into monomer and is configured to the list that concentration is 5~90%Liquid solution, keeping body is tied up in certain temperature, does not make local temperature too high;

    It is 1 to add with monomer weight ratio;

    10000~1;

    10 crosslinking agent and 1;

    10000~1;

    10 initiator, is dissolved in solution;



    2)

    Reaction vessel is put into 0.5~5min of heating in 70~90 DEG C of water-bath, while magnetic agitation, or reaction is heldDevice is put into 0.5~10min in micro-wave oven, and rotates, and uniform heating, reaction is taken out immediately after terminating, and is slowly dropped to room temperature;



    3)

    It is put into 1~6h of reaction in 40~80 DEG C of baking ovens;



    4)

    Temperature is raised again to 85~140 DEG C, continues to react 10~90min, reaction is taken out immediately after terminating, and is slowly dropped to roomTemperature, obtains gel super absorbent resin;



    5)

    Gel super absorbent resin is shredded, placed into open-top receptacle, dries, obtains in 100~150 DEG C of baking ovensSuper absorbent resin, size-reduced machine is crushed, and 180 mesh sieve for subsequent use;

    2)

    The preparation of High water retention microbe mediumIt is 1 by mass ratio;

    300~1;

    900 super absorbent resin and water are placed in culture dish after being configured to resin gel, sterilizingIn, pave, as bottom, will be covered in as top layer on resin gel, had after plain agar medium sterilization afterwardsThe microbiological culture media of the high-moisture-retention of double-layer structure up and down;

    Or by mass percentage, by 0.5~5% nutritional ingredient, 0.1~0.9% super absorbent resin and 94.1~99.4% water is placed in culture dish after being configured to resin gel, sterilizing, is paved, as lower floor, afterwards by plain agar culture mediumIt is covered in after sterilizing on resin gel as upper strata, obtains the microbiological culture media with the high-moisture-retention of double-layer structure up and down;

    Or according to mass percent meter, by 0.5~5% agar powder and 93.5~99.4% water heater configuration into agar solutionAfterwards, in the state of keeping temperature add 0.1~1.5% resin, continue heat, be configured to after resin gel, sterilizing be placed in cultureIn ware, pave, as bottom, will be covered in as top layer on resin gel, had after plain agar medium sterilization afterwardsThe microbiological culture media of the high-moisture-retention of double-layer structure above and below having;

    Or according to mass percent meter, by 0.5~5% nutritional ingredient, 0.5~5% agar powder and 88.5~98.9% waterHeater configuration continues to heat, configures resin into the resin that 0.1~1.5% is added after agar solution, in the state of keeping temperatureIt is placed in culture dish, paves after gel, sterilizing, as bottom, resin gel will be covered in after plain agar medium sterilization afterwardsAs top layer on glue, the microbiological culture media with the high-moisture-retention of double-layer structure up and down is obtained;

    Or add 0.5~5% nutritional ingredient, 0.5~5% agar powder and 88.5~98.9% water according to mass percentHeat is configured to after agar solution, and 0.1~1.5% resin is added in the state of keeping temperature, is continued to heat, is configured to gel and goes outIt is placed in culture dish, paves after bacterium, obtains the microbiological culture media with high-moisture-retention;

    Or according to mass percent meter, by 0.5~5% nutritional ingredient, 0.1~0.9% resin and 94.1~99.4% waterIt is placed in culture dish, paves after being configured to resin gel, sterilizing, obtains the microbiological culture media with high-moisture-retention.

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