Methods and compositions for DNA fragmentation and tagging by transposases

Methods and compositions for DNA fragmentation and tagging by transposases

  • CN 102,796,728 A
  • Filed: 05/23/2012
  • Published: 11/28/2012
  • Est. Priority Date: 05/23/2011
  • Status: Active Grant
First Claim
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1. the in vitro method that is used for the dna fragmentationization of target dna molecular, said method comprises:

  • Make;

    First dna molecular that comprises at least one double-stranded part,Wherein said double-stranded part comprises the recognition sequence of transposase and the part that this dna molecular also comprises the recognition sequence of this transposase;

    The 2nd DNA branch that comprises at least one double-stranded part,Its double center chain partly comprises the recognition sequence of above-mentioned transposase and the part that this dna molecular also comprises this transposase recognition sequence, and the non-identification division of wherein said sequence is different from the non-identification division of first dna molecular;

    WithAbove-mentioned transposaseCombination;

    The combination of wherein above-mentioned three kinds of materials allows them to interact;

    AndMake these interactional materials and target dna molecular and Mg + 2Ion, Mn + 2Ion or Mg + 2Ion and Mn + 2Ion both making interactional material and target DNA associate and cutting through the cutting of transposase-mediation under the condition of target dna molecular and make up, so that the DNA product of cutting to be provided.

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