Process for extracting liver cell growth factor from suckling lull liver

Process for extracting liver cell growth factor from suckling lull liver

  • CN 1,028,002 C
  • Filed: 06/30/1992
  • Issued: 03/29/1995
  • Est. Priority Date: 06/30/1992
  • Status: Active Grant
First Claim
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1. , a kind of production technology of extracting hepatocyte growth factor in the newborn bull liver, (1) selects the certified milk bull, gets Hepar Bovis seu Bubali after slaughtering;

  • (2) with Hepar Bovis seu Bubali repeatedly with normal saline flushing, cut and smash to pieces, (3) are placed in the water-bath heating 30 minutes, temperature is controlled at 95-100 ℃

    , just goes into after the filter in the centrifuge (4000 rev/mins) centrifugal about 30 minutes, it is characterized in that (4) get supernatant;

    Adopt 20000D molecular sieve ultrafiltration two times, (5) degerming, the packing lyophilizing, (6) quality control comprises a, measuring protein content should be at 5mg/ml, b, biological activity detect should the 4-5 of normal control value doubly more than.

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