The mass spectrography of steroid in multiple sample

The mass spectrography of steroid in multiple sample

  • CN 102,812,356 B
  • Filed: 12/09/2010
  • Issued: 08/08/2017
  • Est. Priority Date: 12/11/2009
  • Status: Active Grant
First Claim
Patent Images

1. the method for the amount of the steroid in each test specimen of multiple test specimens is determined with single mass spectroscopy,Wherein the steroid is identical in each test specimen before treatment, and methods described includes:

  • Multiple test specimens are treated differently to form the sample of multiple processing, wherein the processing includes making each test specimenDifferent Cookson type derivating agents are undergone under conditions of the steroid derived from suitable for generation Cookson types, it is describedCookson types derivating agent be selected from 4- phenyl -1,2,4- triazoline -3,5- diketone (PTAD), 4- methyl isophthalic acids, 2,4- triazoline -3,5- diketone (MTAD), 4- [2- (6,7- dimethoxy-4 '"'"'s-methyl -3- oxo -3,4- dihydro-quinoxalines base) ethyl] -1,2,4- threeOxazoline -3,5- diketone (DMEQTAD), 4- (4- nitrobenzophenones) -1,2,4- triazoline -3,5- diketone (NPTAD) and 4- ferroceneYlmethyl -1,2,4- triazolines -3,5- diketone (FMTAD), and wherein due to the processing, the institute in the sample each handledState the steroid that steroid can be different from by mass spectrography in the sample of other processing;

    Merge the sample of the processing to form multiple sample;

    Make the multiple sample suitable for undergoing ionization under conditions of producing one or more by the detectable ion of mass spectrographySource, the ion that one or more of which is produced from the steroid of the sample from each processing is different from a kind of or manyPlant the ion produced from the steroid of the sample from other processing;

    The amount of the ion of the steroid of one or more samples from each processing is detected by mass spectrography;

    WithMake in the amount and each test specimen of ion of the steroid of one or more samples from each processingThe amount of the steroid is associated.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×