The separation of insoluble target protein

The separation of insoluble target protein

  • CN 102,844,326 B
  • Filed: 03/30/2011
  • Issued: 09/29/2017
  • Est. Priority Date: 03/31/2010
  • Status: Active Grant
First Claim
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1. purifying the method for insoluble target silk-fibroin from complete or broken host cell suspension, it comprises the following steps:

  • A) complete or broken host cell suspension comprising insoluble target silk-fibroin and insoluble host cell part is provided,B) aqueous solution of at least one alkali is added in the suspension, wherein the amount of the alkali is enough to crush the host cellAnd/or the insoluble host cell part is dissolved, andC) the insoluble target silk-fibroin is purified from the insoluble host cell part of the dissolving,At least 80% insoluble host cell part is dissolved wherein in step b),Wherein described insoluble target silk-fibroin keeps insoluble in whole process of purification, andWherein described at least one alkali is metal hydroxides or ammonia.

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