Method for separating target cell

Method for separating target cell

  • CN 102,892,900 B
  • Filed: 03/01/2011
  • Issued: 10/31/2017
  • Est. Priority Date: 03/02/2010
  • Status: Active Grant
First Claim
Patent Images

1. it is a kind of be used for sample in tumour cell enrichment and/or separation method, the sample be include red blood cell and/orHematoblastic sample, methods described includes:

  • (a) sample is filtered by the filter element in the hole with size between 0.5 μ

    m and 5 μ

    m;

    (b) make to be contacted with separating surface by the cell that the filter element in step (a) retains, wherein the separation surface isHydrogel on backing material is coated, wherein, the hydrogel includes polycarboxylate polymer or gathered by polycarboxylateCompound is constituted, and wherein, the separation surface includes binding selectively to the affinity molecule of the tumour cell, and wherein, instituteState affinity molecule and be fixed to the hydrogel coating;

    (c) under conditions of allowing the affinity molecule to be combined with the tumour cell, the cell and the separation table are incubatedFace;

    And(d) the separation surface is separated with any uncombined cell and material.

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