Rapid propagation method for dendrocalamus giganteus tissue culture seedling industrial production

Rapid propagation method for dendrocalamus giganteus tissue culture seedling industrial production

  • CN 102,919,124 A
  • Filed: 11/11/2012
  • Published: 02/13/2013
  • Est. Priority Date: 11/11/2012
  • Status: Active Application
First Claim
Patent Images

1. an imperial bamboo group is trained seedling suitability for industrialized production quick-breeding method, it is characterized in that step is as follows:

  • (1) processes outer collection and early stage of growing body;

    gather the upgrowth situation good health without the annual imperial bamboo branch of damage by disease and insect, prune away except secondary branches and leaves, be placed on 4~

    8 ℃

    of Refrigerator stores 1~

    2 day;

    Then by one section of each ring it is whittled into segment, eliminates again the stalk sheaths of bamboo shoots on the ring, and wipe a dirt on stalk surface with gauze, clean with sterile water at last;

    The imperial bamboo branch section that (2) will wash carries out disinfecting for three times under aseptic condition;

    clean with aseptic water washing after at first cleaning with the ethanol of concentration 75%;

    Then imperial bamboo branch section is rendered in the mercuric chloride aqueous solution of concentration 0.1~

    1% in the sterilizing bottle and carried out the 10~

    20min that sterilizes the second time;

    Sterilize for the third time in the aqueous sodium hypochlorite solution with imperial bamboo branch section input concentration 1.5~

    5% again;

    Use at last aseptic water washing 3~

    5 times;

    (3) inducing of axillalry bud;

    will insert in the bud inducing culture through the imperial bamboo branch section of sterilization and induce cultivation, cultivation temperature is 25 ℃

    , first dark cultivation 3~

    5 days, and then carry out illumination cultivation, intensity of illumination is 1000~

    1200Lx;

    Described inducing culture is 1/2MS, wherein contains 6-BA 5.0~

    10.0mg/L, 2,4-D 2.0~

    5.0mg/L, NAA 0.5~

    1.5mg/L, sucrose 10~

    20mg/L and agar 3.5~

    5.0g/L;

    (4) inducing clumping bud is cultivated;

    with imperial bamboo branch section under the lateral bud cutting of inducing cultivation stage to derive, be inoculated into and carry out illumination cultivation in the inducing clumping bud medium, intensity of illumination is 1000~

    1200Lx, cultivates 20~

    25 days under 25 ℃

    of conditions, turns out a large amount of Multiple Buds thereby induce;

    Described inducing clumping bud medium is 3/4MS, wherein contains 6-BA 2.5~

    5.5mg/L, KT1.0~

    2.0mg/L, NAA 0.5~

    1.5mg/L, sucrose 10~

    20mg/L and agar 3.5~

    5.0g/L;

    (5) shoot proliferation is cultivated;

    the previous step inducing clumping bud is cultivated formed Multiple Buds cutting and is divided into budlet clump with 2~

    3 budlets and is inoculated in and carries out shoot proliferation on the proliferated culture medium and cultivate, intensity of illumination is 1000~

    1200Lx, cultivates 20~

    25 days under 25 ℃

    of conditions;

    So that the increase of the existing overall quantity of imperial bamboo Multiple Buds also has the growth of Individual Biomass;

    The shoot proliferation medium is MS, wherein 6-BA 2.0~

    4.0mg/L, KT0.5~

    1.5mg/L, NAA 0.5~

    1.5mg/L, sucrose 10~

    20mg/L and agar 3.5~

    5.0g/L;

    (6) strong sprout propagation is cultivated;

    the Multiple Buds cutting that shoot proliferation is cultivated is divided into budlet clump with 2~

    3 budlets and is inoculated in and carries out strong seedling culture on the proliferated culture medium in strong sprout, intensity of illumination is 1000~

    1200Lx, cultivate 10~

    15 days under 25 ℃

    of conditions, so that the seedling of growing thickly that shoot proliferation is turned out looks more healthy and strong;

    Strong sprout, proliferated culture medium was MS, wherein 6-BA 0.5~

    1.5.0mg/L, KT1.0~

    1.5mg/L, NAA 0.5~

    1.5mg/L, sucrose 10~

    20mg/L and agar 3.5~

    5.0g/L;

    (7) root induction is cultivated;

    the Multiple Buds cutting that strong sprout, propagation was cultivated is divided into budlet clump with 2~

    3 budlets is inoculated in and carries out culture of rootage on the root induction medium, intensity of illumination is 1000~

    1200Lx, cultivating 15~

    20 days rooting rates under 25 ℃

    of conditions is 90%, cultivating 25~

    35 days rooting rates is 95%, described root induction medium is 1/2MS, wherein contains 6-BA 0.1~

    0.5mg/L, NAA 1.5~

    5.0mg/L, IAA 1.5~

    3.0 mg/L, sucrose 10~

    20mg/L and agar 3.5~

    5.0g/L;

    (8) practice seedling and field production;

    the seedling of taking root is placed under the room temperature 3~

    5 days, clean medium, then use the carbendazim aqueous solution soaking 5~

    10min of 1000 times of dilutions, being transplanted in mass ratio, example is in the matrix of 30% humus soil, 20% river sand and 50% earth again, be that 50~

    70% the network control light that shelters from heat or light is to keep ground moistening with obscurity, temperature is controlled at 23~

    28 ℃

    , treats that little bamboo seedling grows to 15~

    20cm when high, moves on to carry out the land for growing field crops in the nursery and cultivate on the spot.

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