A kind of method of recombinant expressed production human thymosin in yeast

A kind of method of recombinant expressed production human thymosin in yeast

  • CN 102,925,470 B
  • Filed: 11/13/2012
  • Issued: 07/06/2016
  • Est. Priority Date: 11/13/2012
  • Status: Active Grant
First Claim
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1. the method producing human thymosin, described method comprises the steps:

  • (1), on the premise of keeping respective gene codon reading frame constant, at coding human seralbumin eggThe both sides of white partial gene sequence and human thymosin gene introduce restriction enzyme enzyme recognition site, then pass through enzymeCut operation and form the fusion sequence with following structure;

    A-X-C, wherein part A is encoding mature human serumAlbumin N-end 1-150~

    the nucleotide sequence of 1-372 amino acids, X is that coding comprises enterokinase or tobacco erosionLine virus protein is digested the nucleotide sequence connecting peptide in site, and C is people'"'"'s thymosin gene;

    (2), above-mentioned A-X-C fusion sequence is connected to expression vector, expression vector is converted and importsIn saccharomycete, obtain recombination engineering bacteria through screening;

    (3), high density fermentation recombinant yeast, through abduction delivering obtain solubility human serum albumins-Thymic peptide fusion protein as one, adds enterokinase or tobacco etch virus protease is cut, and separated purifying obtains recombined humanThymic peptide.

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