High level human diploid cell cultivates varicella-zoster vaccine virus method

High level human diploid cell cultivates varicella-zoster vaccine virus method

  • CN 103,074,304 B
  • Filed: 01/28/2013
  • Issued: 01/13/2016
  • Est. Priority Date: 01/28/2013
  • Status: Active Grant
First Claim
Patent Images

1. high-level human diploid cell cultivates a varicella-zoster vaccine virus method, it is characterized in that:

  • comprise the following steps;

    (1) set up host cell storehouse;

    according to US FDA standard, get ATCCCCL171 cell strain, be initiated with for the 17th generation, be total to 3-5 Secondary Culture through about 20 days, the cell freeze pipe of results is at Liquid nitrogen storage;

    (2) working cardial cell storehouse cell cultures;

    cell step (1) obtained is altogether 3-5 the Secondary Culture through 12-15 days again, carries out and Experiment Data Records according to preparing clinical experiment material requirements, liquid nitrogen and deeply freeze preservation;

    (3) for ensureing the high titre of vaccine virus, carrying out diploid cell population doubling level Simultaneous Monitoring simultaneously, selecting the cell strain of diploid cell population doubling level more than 2.5 to produce vaccine.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×