Method of isolating purified RNA with reduced DNA contaminations

Method of isolating purified RNA with reduced DNA contaminations

  • CN 103,080,311 A
  • Filed: 09/06/2011
  • Published: 05/01/2013
  • Est. Priority Date: 09/06/2010
  • Status: Active Application
First Claim
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1. method of isolation of RNA at least from the sample that contains RNA and DNA, described method comprises:

  • A) in sample, add the acid denaturation composition that comprises chaotropic agent and phenol;

    B) add water-fast organic solvent and separating obtained phase, therefore form comprise water, optional in the middle of mutually and the multiphase mixture of organic phase, wherein RNA is gathered in described water and DNA and protein aggregation in described organic phase and/or mutually middle;

    AndC) from the described RNA of described aqueous phase separation,Wherein add at least a cationic detergent in described each phase front of final separation.

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