The medical composition of prevention or treatment amyloid beta related disease or situation

The medical composition of prevention or treatment amyloid beta related disease or situation

  • CN 103,156,867 B
  • Filed: 12/17/2012
  • Issued: 04/30/2019
  • Est. Priority Date: 12/16/2011
  • Status: Active Grant
First Claim
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1. a kind of preparation method of the medical composition of the benzyl carbinol glycosides only containing acteoside and Isoacteoside, whereinIsoacteoside is 4:

  • 1 to 2;

    3 to the weight ratio of acteoside, and the preparation method comprises the steps of;

    A) fleshy stem of Cistanche tubulosa is added with one first polar solvent and gives extraction;

    B) the step a) extract obtained is added in the column equipped with hydrophobic macrovoid polymer after concentration, so that benzyl carbinol glycosidesAbsorption is on polymer;

    C) the second polar solvent, which is used, as mobile phase flowing lotion elutes the column to remove free compound, and it is substantially most ofBenzyl carbinol glycosides still adsorb on polymer;

    AndD) benzyl carbinol glycosides are eluted with third polar solvent from polymer, obtain the eluent containing benzyl carbinol glycosides,In the first polar solvent be water, methanol, ethyl alcohol, the mixed solvent or water of water and methanol and ethyl alcohol mixed solvent;

    Second polaritySolvent is water;

    And third polar solvent is the mixed solvent of methanol, ethyl alcohol, the mixed solvent or water of water and methanol and ethyl alcohol, andThird polar solvent polarity compared with the second polar solvent is lower;

    E) the eluent concentration containing benzyl carbinol glycosides for obtaining step d) is re-dissolved in water, obtained aqueous solution and a macroporeResin contacts so that benzyl carbinol glycosides therein are adsorbed on macroreticular resin;

    AndF) sequentially the macroreticular resin is eluted respectively with a quadripolarity solvent and one the 5th polar solvent, wherein the 5thThe polarity of polar solvent is low compared with the quadripolarity solvent, so that not contained with eluent obtained by quadripolarity solvent elutionActeoside and Isoacteoside, and with eluent obtained by the elution of the 5th polar solvent only include acteoside withIsoacteoside;

    Wherein the quadripolarity solvent is 25~35% ethanol waters, and the 5th polar solvent be then 35~45% ethanol water.

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