Vitro hepatic differentiation

Vitro hepatic differentiation

  • CN 103,180,436 B
  • Filed: 08/25/2011
  • Issued: 09/22/2017
  • Est. Priority Date: 08/25/2010
  • Status: Active Grant
First Claim
Patent Images

1. a kind of method for being used to induce liver to break up, including:

  • (i) colony of many competent (iPS) cells of induction is provided,(ii) colony is cultivated in entoderm inducing culture, to produce the group of front layer deterministic type entoderm (ADE) cellBody,Wherein, the entoderm inducing culture is the culture medium that chemical composition is determined, it includes fibroblast growth factor;

    Stimulate the TGF beta ligands of the signal path of SMAD2 and SMAD3 mediations;

    Bone morphogenetic protein;

    Phosphatidyl-inositol 3-kinase(PI3K) inhibitor and glycogen synthase kinase-3beta (GSK3 β

    ) inhibitor;

    And(iii) colony of ADE cells is cultivated in liver inducing culture, wherein the ADE cells break up in the culture mediumTo produce the colony of hepatic progenitor cells,Wherein, the liver inducing culture is the culture medium that chemical composition is determined, it is by Essential Chemistry culture medium and stimulates SMAD2With the TGF beta ligands composition of the SMAD3 signal paths mediated.

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