Specific expression promoter of late development stage of plant pollens and use thereof

Specific expression promoter of late development stage of plant pollens and use thereof

  • CN 103,261,417 A
  • Filed: 11/02/2012
  • Published: 08/21/2013
  • Est. Priority Date: 11/07/2011
  • Status: Active Application
First Claim
Patent Images

1. Claimsa kind of DNA sequence dna of separation, it is characterised in that the DNA of separation sequence is selected from one of following group sequence:

  • (a) there is SEQ ID N0;

    Sequence shown in 1;

    (b) under strict conditions can be with(

    A) DNA sequence dna of the DNA hybridization of the sequence;

    (c) SEQ ID NO are included;

    The DNA sequence dna of at least 100 continuous nucleotides in 1;

    With(d) with(

    A) DNA sequence dna of-(c) any sequence complementation.2.-kind of expression cassette, it is characterised in that the expression cassette includes the DNA sequence dna described in claim 1.3. a kind of expression vector, it is characterised in that the expression vector includes the expression cassette described in claim 2.4. a kind of engineering bacteria, it is characterised in that the engineering bacteria contains the expression vector described in claim 3.5. a kind of method of the expressing heterologous nucleotide sequence in plant, methods described includes importing DNA construct to plant, the DNA construct contains promoter and is operatively connected to the purpose heterologous nucleotide sequence of the promoter, wherein the nucleotide sequence of the promoter is selected from one of following group sequence:

    (a) there is SEQ ID NO;

    Sequence shown in 1;

    (b) under strict conditions can be with(

    A) DNA sequence dna of the DNA hybridization of the sequence;

    (c) SEQ ID NO are included;

    The DNA sequence dna of at least 100 continuous nucleotides in 1;

    With(d) with(

    A) DNA sequence dna of-(c) any sequence complementation.6. the method described in claim 5, wherein described plant is monocotyledon.7. the method described in claim 6, wherein described monocotyledon is grass.8. the method described in claim 7, wherein described grass is paddy rice or wheat.9. the method described in claim 5, wherein described heterologous nucleotide sequence refers to the sequence not being operatively connected under native state with the promoter sequence, can be homologous or heterologous for plant host.10. the method described in claim 5, wherein described heterologous nucleotide sequence can be structural gene, regulatory gene, the antisense gene of structural gene, the antisense gene of regulatory gene or the tiny RNA that can interfere with endogenous gene expression, and it can adjust the fertility and pollen germination of pollen in the specific expressed of pollen development late period..11. the DNA sequence dna described in claim 1 is following(

    A) extremely(

    Any one of d) application in:

    (a) plant variety or strain are cultivated;

    (b) Pollination Fertilization ability enhancing plant variety or strain are cultivated;

    (c) plant variety or strain that Pollination Fertilization ability is slackened are cultivated;

    Claims(d) male sterile plants kind or strain are cultivated.2. the application described in claim 11, it is characterised in that described plant is monocotyledon.3. the application described in claim 12, wherein described monocotyledon is grass.4. the method described in claim 13, wherein described grass is paddy rice or wheat.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×