P-hydroxy phenylalanine urine detection reagent and preparation method thereof

P-hydroxy phenylalanine urine detection reagent and preparation method thereof

CN
  • CN 103,323,452 B
  • Filed: 06/21/2013
  • Issued: 07/09/2014
  • Est. Priority Date: 06/21/2013
  • Status: Active Grant
First Claim
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1. a preparation method for para hydroxybenzene alanine urine detection reagent, is characterized in that:

  • This urine detection reagent is by Hg 22+, Hg 2+, Ni 2+, alpha-nitroso-beta-naphthol is dissolved in respectively in corresponding acid solution, after then mixing in proportion, make;

    Described mercuric sulfate is as Hg 2+donor be dissolved in sulfuric acid solution, mercurous nitrate is as Hg 22+donor be dissolved in salpeter solution, nickelous sulfate is as Ni 2+donor be dissolved in distilled water, alpha-nitroso-beta-naphthol is dissolved in salpeter solution;

    The method comprises the following steps;

    1., A liquid preparation;

    18g mercuric sulfate is joined in the sulfuric acid solution of 6mol/L after stirring and dissolving, then being diluted to 100ml with the sulfuric acid solution of 6mol/L, to obtain water white liquid stand-by;

    2., B liquid preparation;

    65g mercurous nitrate is joined in the salpeter solution of 12mol/L, be diluted to 100ml with 12mol/L salpeter solution after dissolving, obtain green liquid, place after solution green is taken off, can use;

    3., C liquid preparation;

    nickelous sulfate 3g is dissolved in distilled water, then with distilled water diluting to 100ml, obtain blue-green liquid, stand-by;

    4., D liquid preparation;

    2g alpha-nitroso-beta-naphthol is dissolved in the salpeter solution of 50ml, 6mol/L, obtains D liquid stand-by;

    5., mix;

    by above-mentioned 1., 2., 3., A, the B, C, D, the four kinds of liquid that make in are 4. after mix 1~

    1.5;

    1.2~

    1.8;

    0.2~

    0.4;

    0.05~

    0.1 by volume, obtain target product para hydroxybenzene alanine urine detection reagent.

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