Preparation method of enzyme-containing compound probiotic plant ferment able to improve gastrointestinal functions

Preparation method of enzyme-containing compound probiotic plant ferment able to improve gastrointestinal functions

  • CN 103,393,121 A
  • Filed: 06/17/2013
  • Published: 11/20/2013
  • Est. Priority Date: 06/17/2013
  • Status: Active Grant
First Claim
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1. one kind is improved gastrointestinal function, contains the preparation method of enzyme compound probiotic plant enzyme, it is characterized in that concrete steps are as follows:

  • 1) clean raw material,2) raw material extracts, get raw material and be placed in extractor, add water to 10 times of raw material weight more than amount, raw material all is immersed in water, heating water temperature to 60 ℃

    , soak 6-10 hour, be heated to again more than 100 ℃

    , extract after 30-50 minute, with extracting liquid filtering out, in the 1;

    8 ratio again extracting in water once, extracted twice liquid merges;

    described raw material is;

    Fructus Hordei Germinatus 20-30 gram, rice sprout 20-30 gram, Hericium erinaceus 20-30 gram, hawthorn 10-20 gram, Chinese yam 20-30 gram, dried orange peel 10-20 gram, Buddha'"'"'s hand 10-15 gram, fructus amomi 10-12 gram, coix seed 20-30 gram, Poria cocos 10-15 gram, matrimony vine 10-20 gram, cape jasmine 5-10 gram, radix polygonati officinalis 10-15 gram, purslane 20-30 gram, Semen Lablab Album 20-30 gram, Gorgon fruit 20-30 gram, Radix Glycyrrhizae 5-10 gram, lotus seeds 10-12 gram, sealwort 10-30 gram, radish seed 6-10 gram, the membrane of a chicken'"'"'s gizzard 8-10 gram, fructus alpiniae oxyphyllae 3-10 gram,3) with step

         2) merging extract normal temperature, normal pressure, hot reflux force to follow bad, be concentrated into proportion 1.06-1.08;

    4) with the concentrate of step

         3), add 5-8% brown sugar, 2% peptone blending, 105 ℃

    of high-temperature sterilizations 40 minutes, filter, and filtrate is nutrient solution;

    5) cooling, inoculation, be cooled to 30-32 ℃

    with the nutrient solution of step

         4) in fermentation tank, connects the compound probiotic kind, mixed culture fermentation,Described compound probiotic kind is saccharomyces cerevisiae, Lactobacillus delbrueckii breast subspecies, Lactobacillus plantarum, lactobacillus gasseri and acetobacter;

    Described bacterial classification inoculum concentration is the 5%-10% of nutrient solution;

    6) fermentation, the fermentation minute fourth phase,The first phase, the oxygen supply yeast phase, oxygen supply 250-300L/min, the fermentation tank pressure-controlled, at 0.07~

    0.08MPa, keeps room temperature 22-25 ℃

    , fermentation temperature 30-32 ℃

    , time 3-7 days,The second phase, acetobacter participates in acid process, fermentation temperature 30-32 ℃

    , and time 5-7 days,The third phase, the lactobacter growth animated period, micro-aerobe fermentation, 1.2L/min, 37 ℃

    of fermentation temperatures, time 5-7 days,The fourth phase, 28 ℃

    -30 ℃

    of fermentation temperatures, observed seven days, allows saccharomycete, acetobacter and lactobacter growth breeding be in relative balance state;

    7) quality detecting index is packaged into finished product after requiring detection qualified according to product standard.

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