Preparation method of enzyme-containing compound probiotic plant ferment able to improve gastrointestinal functions

Preparation method of enzyme-containing compound probiotic plant ferment able to improve gastrointestinal functions

  • CN 103,393,121 B
  • Filed: 06/17/2013
  • Issued: 08/06/2014
  • Est. Priority Date: 06/17/2013
  • Status: Active Grant
First Claim
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1. improve a gastrointestinal function, containing the preparation method of enzyme compound probiotic plant enzyme, it is characterized in that concrete steps are as follows:

  • 1) clean raw material,2) raw material extracts, get raw material and be placed in extractor, add water to 10 times of raw material weight more than amount, raw material is all immersed in water, heating water temperature to 60 DEG C, soak 6-10 hour, be heated to again more than 100 DEG C, extract after 30-50 minute, by extracting liquid filtering out, in 1;

    8 ratio again extracting in water once, extracted twice liquid merge;

    Described raw material is;

    Fructus Hordei Germinatus 20-30 gram, rice sprout 20-30 gram, Hericium erinaceus 20-30 gram, hawthorn 10-20 gram, Chinese yam 20-30 gram, dried orange peel 10-20 gram, Buddha'"'"'s hand 10-15 gram, fructus amomi 10-12 gram, coix seed 20-30 gram, Poria cocos 10-15 gram, matrimony vine 10-20 gram, cape jasmine 5-10 gram, radix polygonati officinalis 10-15 gram, purslane 20-30 gram, Semen Lablab Album 20-30 gram, Gorgon fruit 20-30 gram, Radix Glycyrrhizae 5-10 gram, lotus seeds 10-12 gram, sealwort 10-30 gram, radish seed 6-10 gram, the membrane of a chicken'"'"'s gizzard 8-10 gram, fructus alpiniae oxyphyllae 3-10 gram,3) by step

         2) merging extract normal temperature, normal pressure, hot reflux is forced to follow badly, is concentrated into proportion 1.06-1.08;

    4) by step

         3) concentrate, add 5-8% brown sugar, 2% peptone blending, 105 DEG C of high-temperature sterilizations 40 minutes, filter, filtrate is nutrient solution;

    5) cooling, inoculation, by step

         4) nutrient solution in fermentation tank, be cooled to 30-32 DEG C, connect compound probiotic kind, mixed culture fermentation,Described compound probiotic kind is saccharomyces cerevisiae, Lactobacillus delbrueckii subsp. lactis, Lactobacillus plantarum, lactobacillus gasseri and acetobacter;

    The 5%-10% that described bacterial classification inoculum concentration is nutrient solution;

    6) fermentation, the fermentation point fourth phase,The first phase, oxygen supply yeast phase, oxygen supply 250-300L/min, fermentation tank pressure-controlled, at 0.07~

    0.08MPa, keeps room temperature 22-25 DEG C, fermentation temperature 30-32 DEG C, time 3-7 days,The second phase, acetobacter participates in acid process, fermentation temperature 30-32 DEG C, and time 5-7 days,The third phase, lactobacter growth animated period, micro-aerobe fermentation, 1.2L/min, 37 DEG C of fermentation temperatures, time 5-7 days,The fourth phase, 28 DEG C-30 DEG C of fermentation temperatures, observe seven days, allow saccharomycete, acetobacter and lactobacter growth breed in relative balance state;

    7) quality detecting index is packaged into finished product after requiring detection qualified according to product standard.

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