Methods to determine zygosity in a bulked sample

Methods to determine zygosity in a bulked sample

  • CN 103,403,185 A
  • Filed: 12/28/2011
  • Published: 11/20/2013
  • Est. Priority Date: 12/29/2010
  • Status: Active Application
First Claim
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1. the existence of the nucleotide sequence that in definite nucleic acid, specific insertion point place inserts or the method for shortage, the method comprises:

  • From described sample separation nucleic acid;

    Described nucleic acid is contacted with reverse primer with forward primer, and described forward primer can be in conjunction with the nucleic acid of described insertion point upstream;

    Described reverse primer can be in conjunction with the nucleic acid in described insertion point downstream;

    Use the nucleic acid of described primer to regenerate between described primer;

    AndWhether the nucleic acid of analyzing regeneration exists to determine that the nucleotides sequence that inserts is listed in described sample.

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