A kind of method using the mixed sugar segmentation containing pentose and hexose, mixed fungus fermentation production ethanol, butanol and acetone

A kind of method using the mixed sugar segmentation containing pentose and hexose, mixed fungus fermentation production ethanol, butanol and acetone

  • CN 103,409,470 B
  • Filed: 05/15/2013
  • Issued: 10/17/2017
  • Est. Priority Date: 05/15/2013
  • Status: Active Grant
First Claim
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1. a kind of method using the mixed sugar segmentation containing pentose and hexose, mixed fungus fermentation production ethanol, butanol and acetone, itsIt is characterised by comprising the following steps:

  • A, the mixed sugar containing pentose and hexose is made into total sugar concentration for 20g/l~100g/l, and pentose accounts for the quality of total reducing sugarPercentage is 5~65%, adds nitrogen source and phosphorus source obtains fermentation medium, the quality of the nitrogen source and phosphorus source is fermented and culturedThe 10~50% of total reducing sugar quality in base, then obtain boiling mash by fermentation medium boiling sterilization;

    B, treat boiling mash be cooled to less than 42 DEG C be segmented, mixed fungus fermentation, saccharomyces cerevisiae or acetone-butanol shuttle are accessed firstOne kind in bacterium, another bacterial strain among two kinds of bacterial strains of access after fermentation 4~72 hours continues fermentation and obtains karusenLiquid;

    The fermentation is anaerobic fermentation, and fermentation temperature is 25~41 DEG C, 30~168h of total fermentation time;

    C, fermentation liquid filtered, distilled, rectifying obtains final ethanol, butanol, product acetone.

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