Lipoprotein complexes and their preparation and use

Lipoprotein complexes and their preparation and use

  • CN 103,443,123 B
  • Filed: 02/06/2012
  • Issued: 05/29/2020
  • Est. Priority Date: 02/07/2011
  • Status: Active Grant
First Claim
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1. A method of preparing a lipoprotein complex comprising:

  • (a) cooling a starting suspension comprising a lipid component and a protein component from a temperature in a first temperature range comprising a temperature not lower than 5 ℃ and

    not higher than 5 ℃

    the transition temperature of the protein component to a temperature in a second temperature range comprising a temperature not lower than 5 ℃ and

    not higher than 5 ℃

    the transition temperature of the lipid component, wherein;

    (i) the lipid component consists essentially of lipid particles formed by homogenization;

    (ii) the protein component consists essentially of a lipid-binding peptide and/or lipid-binding protein that has not been homogenized;

    (iii) the starting suspension is the product of a process comprising;

    combining a lipid component with a protein component;

    (iv) the starting suspension has a lipid to protein molar ratio of 2;

    1 to 200;

    1; and

    (v) the lipids in the starting suspension comprise neutral phospholipids and negatively charged phospholipids in a weight;

    weight ratio of 99;

    1 to 95;

    5;

    (vi) the acyl chains of neutral and negatively charged phospholipids contain 14 to 20 carbons in length;

    (b) heating the cooled suspension of (a) from a temperature in the second temperature range to a temperature in the first temperature range;

    (c) cooling the heated suspension of (b) from a temperature in the first temperature range to a temperature in the second temperature range; and

    (d) repeating steps (b) and (c) until at least 80% of the protein component is incorporated into the lipoprotein complex, thereby forming the lipoprotein complex.

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