For cultivating the method for microorganism and bioreactor

For cultivating the method for microorganism and bioreactor

  • CN 103,476,245 B
  • Filed: 02/10/2012
  • Issued: 06/12/2018
  • Est. Priority Date: 02/11/2011
  • Status: Active Grant
First Claim
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1. a kind of method of the culture biology in tubulose bioreactor (1), the tubulose bioreactor (1) is comprising extremelyA few pipe (2), circulating slot (3) and gas supply (5), wherein ring of at least one pipe (2) as at least 100 metersCirculating slot (3) is connected to, wherein gas supply (5) is connected at least one pipe (2), at least one pipe (2)It is the plastic tube at most 1mm wall thickness, the described method comprises the following steps:

  • A. provide in the bioreactor (1) fluid nutrient medium (8) containing biology andB. the culture biology in bioreactor (1),It is characterized in that before step b) and/or period is by adding NaOH, Na2CO3And/or NaHCO3By at least 10 mM to mostThe cation of more 100 mM concentration adds to fluid nutrient medium;

    The mobile fluid nutrient medium (8) wherein during at least one period of step b), and in at least one of step b)At least 0.2% volume CO will be contained during period2Gas phase made by gas at least one pipe (2) supply (5)Culture medium is introduced for Bolus-triggered technique (9) or piston flow, wherein the culture medium (8) only supplies (5) introduced described by gasGas phase and move,Wherein described bioreactor (1) has at least one reaction zone, and the reaction zone has at least 0.1 degree to most3.0 degree of gradient andWherein tubulose bioreactor (1) is the system of closing, and wherein gas supply (5) is connected to circulating slot (3)Top and CO2It supplies (6), gas phase is introduced into culture medium at least one pipe (2).

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