Methods for sequencing, amplification and detection of nucleic acids comprising internally labeled primer

Methods for sequencing, amplification and detection of nucleic acids comprising internally labeled primer

  • CN 103,502,475 A
  • Filed: 05/04/2012
  • Published: 01/08/2014
  • Est. Priority Date: 05/06/2011
  • Status: Active Application
First Claim
Patent Images

1. the method for nucleic acid sequencing or amplification and detection, described method comprises the following steps:

  • I) provide at least one nucleic acid primer, the Nucleotide that it comprises at least one mark,Enzyme and reagent ii) are provided, with the nucleic acid primer of described at least one mark, carry out amplifying target nucleic acid,Iii) by reactive component incubation under the condition that is suitable for the target nucleic acid primer amplification,Iv) by the Nucleotide of mark, detect the nucleic acid of amplification,Wherein said nucleic acid primer comprises following sequence;

    5’

    -N aXN b-3’



    Wherein N can be any Nucleotide,Wherein " a " and " b " mean Nucleotide quantity and can be in 1 to 150 scope, and wherein X is the Nucleotide of described at least one mark.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×