Small molecule RNA, preparation method thereof and application in pharmaceuticals for transcriptional activity of specificity up-regulated genes

Small molecule RNA, preparation method thereof and application in pharmaceuticals for transcriptional activity of specificity up-regulated genes

  • CN 103,509,797 A
  • Filed: 10/09/2013
  • Published: 01/15/2014
  • Est. Priority Date: 10/09/2013
  • Status: Active Grant
First Claim
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1. raise native gene and express a preparation method for the microRNA of use, it is characterized in that, comprise the following steps:

  • S1. the target spot of microRNA is determinedWhile containing TATA box sequence in promotor, target site is the scope that upstream and downstream is respectively extended 20 bases centered by TATA box sequence;

    While not containing TATA box sequence in promotor, target site is the sequence of this genetic transcription initiation site upstream 1 ~ 50 base,S2. the design of microRNA and syntheticThe antisense strand of microRNA and target sequence are complementary, obtain core sequence,S3. modify the core sequence of S2 gained, make microRNA comprise following structure;

    Length is that double-stranded siRNA, the length that 19 base 3 '"'"' ends dangle with dTdT is less than or is greater than the double-stranded siRNA of 19 bases, external synthetic Microrna, external synthetic strand or double-stranded sense-rna or external synthetic siRNA, microRNA and sense-rna through chemically modified.

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