Dual variable domain antibodies sample binding protein with exchange area orientation

Dual variable domain antibodies sample binding protein with exchange area orientation

  • CN 103,562,221 B
  • Filed: 03/28/2012
  • Issued: 06/14/2019
  • Est. Priority Date: 03/28/2011
  • Status: Active Grant
First Claim
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1. a kind of antibody sample binding protein comprising forming 4 polypeptide chains of 4 antigen binding sites, wherein 2 polypeptide chain toolsThere is the structure being expressed from the next:

  • VL1-L1-VL2-L2-CL[I]And 2 polypeptide chains have the structure being expressed from the next;

    VH2-L3-VH1-L4-CH1-Fc[II]Wherein;

    VL1For the first immunoglobulin light chain variable structural domain;

    VL2For the second immunoglobulin light chain variable structural domain;

    VH1For the first immunoglobulin heavy chain variable structural domain;

    VH2For the second immunoglobulin heavy chain variable structural domain;

    CLFor immunoglobulin light chain constant structural domain;

    CH1For immunoglobulin CH1Heavy chain constant domain;

    Fc is immunoglobulin hinge region and CH2

    CH3Heavy chain immunoglobulin constant domain;

    WithL1

    L2

    L3And L4For Amino acid linker, have according to the amino acid length combined as follows;

    L1=1, L2=1, L3=2, L4=4;

    L1=1, L2=2, L3=1, L4=4;

    L1=1, L2=2, L3=12, L4=12;

    L1=1, L2=2, L3=15, L4=15;

    L1=1, L2=2, L3=2, L4=2;

    L1=1, L2=2, L3=2, L4=4;

    L1=1, L2=2, L3=2, L4=7;

    L1=1, L2=2, L3=4, L4=4;

    L1=1, L2=2, L3=4, L4=7;

    L1=1, L2=2, L3=6, L4=4;

    L1=1, L2=2, L3=6, L4=7;

    L1=1, L2=2, L3=9, L4=12;

    L1=1, L2=3, L3=2, L4=4;

    L1=1, L2=4, L3=2, L4=4;

    L1=2, L2=2, L3=2, L4=4;

    L1=5, L2=5, L3=1, L4=2;

    L1=7, L2=3, L3=1, L4=2;

    L1=7, L2=5, L3=1, L4=2;

    L1=10, L2=3, L3=1, L4=2;

    L1=10, L2=5, L3=1, L4=2;

    L1=5, L2=5, L3=2, L4=2;

    L1=7, L2=5, L3=2, L4=2;

    L1=10, L2=3, L3=2, L4=2;

    L1=10, L2=5, L3=2, L4=2;

    OrL1=12, L2=6, L3=8, L4=2;

    And wherein the polypeptide of Formulas I and the polypeptide of Formula II form exchange light-heavy chain pair.

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