Transgenic regulation and control miRNA (micro ribonucleic acid) gene algae capable of realizing continuous photosynthetic hydrogen production and creation method of gene algae

Transgenic regulation and control miRNA (micro ribonucleic acid) gene algae capable of realizing continuous photosynthetic hydrogen production and creation method of gene algae

  • CN 103,571,868 A
  • Filed: 11/05/2013
  • Published: 02/12/2014
  • Est. Priority Date: 11/05/2013
  • Status: Active Application
First Claim
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1. continuously photocatalytic closes a construction process for the modulation control miRNAs gene green alga of putting hydrogen, comprises the steps:

  • (1) screening of transgene receptor algae and cultivation;

    (2) screening or design regulation and control miRNAs;

    the miRNAs changing according to expression amount sets up little RNA storehouse, and the target gene that therefrom filters out prediction is relevant to lightsystemⅡ and

    the miRNAs that all significantly raises of expression amount, or according to the green alga target-gene sequence that affect photosynthetic efficiency, design and synthesize the artificial mi RNA s of this gene;

    (3) build respectively and contain the green alga nuclear expression carrier that respectively regulates and controls miRNAs or the chlorophta chlorophyll body expression vector of (2) in steps;

    (4) the green alga nuclear expression carrier obtaining in step (3) or the genetic transformation of chlorophta chlorophyll body expression vector;

    (5) screening of transgenosis green alga.

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