Kit for detecting mutation and/or polymorphism of specific region in target nucleotide sequence

Kit for detecting mutation and/or polymorphism of specific region in target nucleotide sequence

  • CN 103,571,937 A
  • Filed: 09/21/2012
  • Published: 02/12/2014
  • Est. Priority Date: 08/09/2012
  • Status: Active Application
First Claim
Patent Images

1. for detecting a sudden change of one of target nucleotide sequence specific region and/or a test kit for polymorphism, comprising:

  • At least one the first primer, is comprised of one first fragment and one second fragment, and 3 '"'"' end of this first fragment connects 5 '"'"' end of this second fragment,The complementary strand that wherein this first fragment is a First ray, and there is an about 10-30 Nucleotide, and this First ray system is positioned at sequence identification number;

    between 3 position, the 375th 406 of position to the, and must comprise the 401st position, this second fragment is one second sequence again, and there is an about 10-30 Nucleotide, and this second sequence system is positioned at sequence identification number;

    between 3 position, the 320th 348 of position to theOne second primer, wherein this second primer is one the 3rd sequence, and has an about 10-30 Nucleotide, and the 3rd sequence system is positioned at sequence identification number;

    between 3 position, the 298th 328 of position to the;

    At least one three-primer, is comprised of one the 3rd fragment and one the 4th fragment, and 3 '"'"' end of the 3rd fragment connects 5 '"'"' end of the 4th fragment,Wherein the 3rd fragment is one the 4th sequence, and there is an about 10-30 Nucleotide, and the 4th sequence system is positioned at sequence identification number;

    between 3 position, the 396th 428 of position to the, and must comprise the 401st position, the 4th fragment is the complementary strand of one the 5th sequence again, and there is an about 10-30 Nucleotide, and the 5th sequence system is positioned at sequence identification number;

    between 3 position, the 451st 479 of position to the;

    AndOne the 4th primer, wherein the 4th primer is the complementary strand of one the 6th sequence, and has an about 10-30 Nucleotide, and the 6th sequence system is positioned at sequence identification number;

    between 3 position, the 482nd 514 of position to the;

    Wherein this test kit system is for one of detecting in one of sample from an individuality target gene sudden change and/or a polymorphism of specific region, and this target gene is CYP2C9(sequence identification number;

         1), and this specific region is CYP2C9 *2 (rs1799853), and this sudden change or and/or the occurrence positions of polymorphism be positioned at sequence identification number;

    1 the 8633rd position, Wherein this test kit system is used in a polymerase chain reaction again.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×