Improved light-emitting molecule

Improved light-emitting molecule

  • CN 103,582,701 B
  • Filed: 12/05/2011
  • Issued: 08/14/2018
  • Est. Priority Date: 12/03/2010
  • Status: Active Grant
First Claim
Patent Images

1. the method for preparing the copepod luciferase polypeptide of modification comprising modification SEQ ID NO:

  • 792 amino acid sequence,The modification includes being selected from following at least one modification:

    (a) L corresponds to consensus sequence SEQ ID NO at least one;

    905 the 85th or 98 position and it is following in extremelyIt is one few:

    (i) it is selected from following motif:

    In C6RCE, KCA or KCE,In C6RCEE or KCEE,In C6RCED, KCAD or KCED,In C6And/or C10ERC,In C6RC or KC,In C10GRC,In C5And/or C6RCA,In C6RCAE or KCAE,In C5And/or C6RCAD, andIn C9KCSD,Wherein C5

    C6

    C9And C10It is consensus sequence SEQ ID NO;

    905 conservative cysteine;

    AndWherein A is alanine, and R is arginine, and E is glutamic acid, and D is aspartic acid, and G is glycine, and S is serine, and K is badPropylhomoserin;

    And(ii) across the downstream of signal peptide sequence and consensus sequence SEQ ID NO;

    905 the 74th, 75,76,77,78 or 79 ammoniaThe total deletion of the amino acid sequence of the upstream of base acid residue;

    Or(b) it is selected from following motif:

    In C10ERC,In C5RCA,In C5And/or C6RCAD, andIn C9KCSD,Wherein C5

    C6

    C9And C10It is consensus sequence SEQ ID NO;

    905 conservative cysteine;

    AndOptionally further include following at least one:

    (i) L corresponds to consensus sequence SEQ ID NO at least one;

    905 the 85th or 98 position;

    And(ii) across the downstream of signal peptide sequence and consensus sequence SEQ ID NO;

    905 the 74th, 75,76,77,78 or 79 ammoniaThe total deletion of the amino acid sequence of the upstream of base acid residueThus the copepod luciferase polypeptide of modification is generated.

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