Method for preparing polymeric protein composed of monomeric protein produced by fusing protein having immunoglobulin fold structure to protein capable of serving as subunit structure

Method for preparing polymeric protein composed of monomeric protein produced by fusing protein having immunoglobulin fold structure to protein capable of serving as subunit structure

  • CN 103,608,463 A
  • Filed: 06/25/2012
  • Published: 02/26/2014
  • Est. Priority Date: 06/23/2011
  • Status: Active Application
First Claim
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1. the preparation method of multimeric protein, described multimeric protein is by making to have the albumen of immunoglobulin folding structure and can forming the albumen of subunit structure or its functional fragment merges and the monomeric protein that obtains forms, and this preparation method comprises the steps:

  • Step (A), prepares to be the albumen of insoluble particle form in the thalline of microorganism, and this albumen is the monomeric protein that the albumen that makes to have immunoglobulin folding structure and other protein fusions that can form subunit structure obtain;

    Step (B), utilizes the monomeric protein of preparing in the aqueous solution solubilization step (A) that contains lauroyl L-glutamic acid or its salt;

    Step (C), the solution obtaining in dilution step in the damping fluid that contains arginine or arginine derivative (B), the concentration of reduction lauroyl L-glutamic acid or its salt;

    AndStep (D), utilizes the method be selected from following (d1)~

    (d4), and the solvent exchange of the solution obtaining in step (C) is become to damping fluid,(d1) be selected from the column chromatography of gel permeation chromatography, ion exchange chromatography, hydrophobic interaction chromatography and their combination;

    (d2) ultrafiltration process;

    (d3) dialysis method;

    And(d4) (d1)~

    two or more combination in (d3).

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