α -amylase variants

α -amylase variants

  • CN 103,649,307 B
  • Filed: 06/29/2012
  • Issued: 03/27/2020
  • Est. Priority Date: 06/30/2011
  • Status: Active Grant
First Claim
Patent Images

1. An isolated variant of a parent α

  • -amylase, wherein the variant has α

    -amylase activity, and wherein the variant is a substitution of the mature polypeptide of SEQ ID No. 7 at a position corresponding to the position numbered according to SEQ ID No. 1;

    W140Y + N195F + V206Y + Y243FK72R + N195F + V206Y + Y243F + E260H + G273VW140Y + N195F + V206Y + Y243F + E260GN195F + V206Y + Y243F + E260K + W284DW140Y + N195F + V206Y + Y243F + E260G + W284DW140Y + N195F + V206Y + Y243F + E260T + W284DW189E + N195F + V206Y + Y243FW140Y + N195F + V206Y + Y243F + W284DN195F + V206Y + Y243F + G477RN195F + V206Y + Y243F + G477MW140Y + W189G + N195F + V206Y + Y243F + E260GW140Y + N195F + V206Y + Y243F + E260G + G477EW140Y + N195F + V206Y + Y243F + E260G + G476EW140Y + N195F + V206Y + Y243F + E260G + S303GW140Y + W189T + N195F + V206Y + Y243F + E260GW140Y + N195F + V206Y + Y243F + E260G + G337NY100I + W140Y + N195F + V206Y + Y243F + E260GG109A + W140Y + N195F + V206Y + Y243F + E260GW140Y+ N195F+ V206Y+ Y243F+ E260G+ W439RG109A+W140Y+E194D+N195F+V206Y+Y243F+ E260GG109A+W140Y+N195F+V206Y+Y243F+E260G+ G476ET51I+Y100I+G109A+W140Y+N195F+V206Y+ Y243F+ E260GT51I+G109A+W140Y+N195F+V206Y +Y243F+E260G+ W439RT51I+ S52Q+ N54K+ G109A+ W140Y+ N195F+ V206Y+ Y243F+ E260G+ G476EW140Y+N195F+V206Y+Y243F+E260G+G304R+ G476KW140Y+ N195F+ V206Y+Y243F+E260G+W284R+G477KW140Y+N195F+V206Y+Y243F+E260G+W284F+ G477RN195F+V206Y+ Y243F+ E260G+ W284D。

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