Extraction method and antibacterial activity screening method for fat-soluble active ingredients in insects and arthropods

Extraction method and antibacterial activity screening method for fat-soluble active ingredients in insects and arthropods

  • CN 103,655,627 A
  • Filed: 09/07/2012
  • Published: 03/26/2014
  • Est. Priority Date: 09/07/2012
  • Status: Active Application
First Claim
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1. an extracting method for fat-soluble effective ingredient in insecticide and arthropod body, is characterized in that it comprises following steps:

  • Get dried insecticide or arthropod body polypide, at room temperature use dichloromethane lixiviate 2 times, each lixiviate 48-72 hour and be aided with ultrasound procedure;

    Lixiviating solution is through vacuum filtration, merging filtrate, and decompression and solvent recovery, obtains insecticide dichloromethane crude extract;

    The crude extract of gained is placed in to evaporating dish, under 40-50 ℃

    of condition, heating in water bath 6-8h, the dichloromethane volatilization to extractum is complete;

    The volume mixture that crude extract and distilled water are pressed to 1;

    2-4, then transfers in separatory funnel, adds petroleum ether to jolt extraction, and standing rear layering, takes off a layer water, according to aforesaid operations, again extracts 1-2 time, reclaims water;

    After water is concentrated, transfer in evaporating dish to about 40-50 ℃

    oven dry in water-bath, obtain removing fat-soluble effective ingredient in the insecticide of insect wax and arthropod body.

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