Method for identifying antibiotic targets

Method for identifying antibiotic targets

  • CN 103,687,948 A
  • Filed: 05/03/2012
  • Published: 03/26/2014
  • Est. Priority Date: 05/05/2011
  • Status: Active Application
First Claim
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1. for being identified in bacterium the method as the indispensable gene of antibiotic targets, described method comprises step:

  • (a) by use, activate transposon (Tn a) carry out transposon mutagenesis and produce mutant bacterial storehouse, wherein said Tn acomprising promotor makes to insert and be increased in transcribing of insertion point place or near gene insertion point to the transposon in DNA of bacteria;

    (b) under the described antibiotic existence of difference amount, make bacterial growth from mutant strain storehouse to produce two or more test cultures;

    And(c) Tn between compare test culture athe distribution of inserting is usingd and is identified in described bacterium the indispensable gene of inferring as described antibiotic target.

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