Method for detecting the presence of carbapenemase-producing bacteria in a sample

Method for detecting the presence of carbapenemase-producing bacteria in a sample

  • CN 103,764,838 A
  • Filed: 06/21/2012
  • Published: 04/30/2014
  • Est. Priority Date: 06/22/2011
  • Status: Active Grant
First Claim
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1. for detection of there is the method for the bacterium that produces carbapenem enzyme in sample, described method comprises step:

  • A) on test sample, carry out lysis to obtain enzyme suspension;

    B) the enzyme suspension that a part of step is obtained in a) reacts with test kit, and described test kit comprises-carbapenem enzyme substrates, is selected from carbapenem and cephamycin,-pH color indicator, when the pH of reaction mixture is 6.4-8.4, described pH color indicator will change color,Step b wherein) colour-change after represents to exist in described test sample the bacterium that produces carbapenem enzyme.

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