Plasma protein fractionation by sequential polyacid precipitation

Plasma protein fractionation by sequential polyacid precipitation

  • CN 103,797,023 B
  • Filed: 09/14/2012
  • Issued: 04/10/2020
  • Est. Priority Date: 09/16/2011
  • Status: Active Grant
First Claim
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1. A method of separating at least a first and a second protein from a sample of plasma, wherein the first protein is fibrinogen and the second protein is immunoglobulin G, the method comprising the steps of:

  • a) providing a sample of plasma in a container;

    b) adding a polybasic acid and a salt to the plasma, whereby the plasma comprises 50-100mmol/L total salt at a pH between 6 and 8, resulting in the formation of a first protein precipitate comprising a majority of fibrinogen in the sample, and recovering the first protein precipitate and first supernatant, respectively; and

    c) adding an additional amount of polyacid to the first supernatant resulting in the formation of a second protein precipitate comprising a majority of immunoglobulin G in the sample and a second supernatant, and separately recovering the second protein precipitate, wherein the amount of polyacid added results in a total polyacid concentration in the first supernatant of 5-8%;

    wherein the first protein precipitate comprises a concentrate of the first protein and the second protein precipitate comprises a concentrate of the second protein;

    wherein the polyacid is a polymer comprising a functional group that is negatively charged at pH 6 and higher; and

    wherein the salt is selected from the group consisting of sodium phosphate, potassium phosphate, ammonium phosphate, sodium citrate, potassium citrate, ammonium citrate, sodium sulfate, potassium sulfate, ammonium sulfate, sodium acetate, potassium acetate, ammonium acetate, or any combination thereof.

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