Use of g-clamp for improved allele-specificpcr

Use of g-clamp for improved allele-specificpcr

  • CN 103,797,132 A
  • Filed: 09/12/2012
  • Published: 05/14/2014
  • Est. Priority Date: 09/23/2011
  • Status: Active Application
First Claim
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1. the method for the allele specific amplification of target sequence variant, described target exists with the form of several variant sequences, and described method comprises:

  • (a) at least one variant of the first and second oligonucleotide and described target sequence is hybridized;

    One or more variants of wherein said the first oligonucleotide and described target sequence are complementary at least partly, and one or more variants of described the second oligonucleotide and described target sequence are complementary at least partly, and have and at least one selective kernel thuja acid of only a kind of variant complementation of described target sequence;

    Wherein said the second oligonucleotide mixes at least one " G-shaped clamp " Nucleotide;

    (b) extend described the second oligonucleotide with nucleic acid polymerase, while wherein hybridization when described the second oligonucleotide and with the described target sequence variant of described at least one selective kernel thuja acid complementation, described polysaccharase can effectively extend described the second oligonucleotide, and when described the second oligonucleotide with and when the not complementary described target sequence variant hybridization of described at least one selective kernel thuja acid, extend efficiency obviously lower.

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