Method for injecting low-energy N<+> for mutation breeding hericium erinaceus strain and bred strain

Method for injecting low-energy N<+> for mutation breeding hericium erinaceus strain and bred strain

  • CN 103,805,593 A
  • Filed: 02/17/2014
  • Published: 05/21/2014
  • Est. Priority Date: 02/17/2014
  • Status: Active Application
First Claim
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1. a low energy N +the method of injecting mutagenic and breeding Hericium erinaceus (Bull. Ex Fr.) Pers. bacterial strain, is characterized in that:

  • comprise the following steps;

    successively (1) the Hericium erinaceus (Bull. Ex Fr.) Pers. starting strain that mutagenesis is carried out in screening;

    select multiple different Hericium erinaceus (Bull. Ex Fr.) Pers. to produce bacterial classification, carry out Hericium erinaceus culture, select Hericium erinaceus (Bull. Ex Fr.) Pers. bacterial strain that hericium erinaceus fruiting body output the is the highest starting strain as mutagenesis;

    (2) select the protoplastis of Hericium erinaceus (Bull. Ex Fr.) Pers. starting strain as the material of Low energy N+ ions mutagenesis;

    making concentration is 10 5the Hericium erinaceus (Bull. Ex Fr.) Pers. protoplastis suspension of individual/ml level, gets the protoplastis suspension of 0.1ml and coats in sterile petri dish, smoothens air-dry;

    (3) nitrogen ion implantation mutagenesis;

    the material that step (2) is coated in culture dish carries out nitrogen ion implantation mutagenesis immediately, then, with the sucrose solution wash-out with 0.6mol/l, coats on Hericium erinaceus (Bull. Ex Fr.) Pers. regeneration culture medium and cultivates;

    (4) screening of mutagenic strain;

    after bacterium colony grows up to, select sturdy dense, the fast mycelia that grows of mycelia and carry out liquid fermenting test, then carry out the mensuration of hypha biomass, more than 5% bacterial strain is stored in test tube as the mutagenesis bacterial classification after primary dcreening operation higher than starting strain to select biomass;

    Then the bacterial classification obtaining and starting strain are carried out to Hericium erinaceus culture test simultaneously after above-mentioned screening, select biological transformation ratio higher than control strain the mutagenic strain of more than 5% bacterial strain after as multiple sieve;

    (5) stabilization characteristics of genetics checking;

    by 20 generations of bacterial strain continuous passage that obtain after above-mentioned mutagenesis screening, then carry out experiment in cultivation, select stable yield, the proterties bacterial strain without considerable change, be finally defined as for the production of the new bacterial strain of Hericium erinaceus (Bull. Ex Fr.) Pers. high yield.

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