High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris

High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris

  • CN 103,827,290 A
  • Filed: 05/08/2012
  • Published: 05/28/2014
  • Est. Priority Date: 05/20/2011
  • Status: Active Application
First Claim
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1. a method that produces many subunits mixture, it comprises:

  • (a) provide the eukaryotic culture of the gene that comprises the expression that contains the subunit that described many subunits mixture is provided;

    (b) heavy dose of ethanol is added into described culture;

    With(c) cultivate described culture to produce described many subunits mixture.

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