Peanut peptide preparation method for enzymatically decomposing thermal-crushed peanut meal step by step with double neutral protease

Peanut peptide preparation method for enzymatically decomposing thermal-crushed peanut meal step by step with double neutral protease

  • CN 103,865,972 A
  • Filed: 03/20/2014
  • Published: 06/18/2014
  • Est. Priority Date: 03/20/2014
  • Status: Active Application
First Claim
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1. two neutral protease substep enzymolysis hot pressed peanut meals are prepared a method for peanut peptide, it is characterized in that, comprise the following steps:

  • Step 1, hot pressed peanut meal is mixed with distilled water, the hot pressed peanut meal solution that preparation quality concentration is 13~

    15%, and measure the content of Semen arachidis hypogaeae protein in described hot pressed peanut meal;

    Step 2, adjusting to keep the temperature of described hot pressed peanut meal solution be 40~

    50 ℃

    , in described hot pressed peanut meal solution, add neutral protease, carry out enzyme digestion reaction 1.5~

    2.0h, obtain the first enzymolysis solution, the add-on of wherein said neutral protease obtains according to the cubage of Semen arachidis hypogaeae protein in the mass concentration of described hot pressed peanut meal solution and the hot pressed peanut meal measured;

    Step 3, in described the first enzymolysis solution, add compound protease, keep 40~

    50 ℃

    of temperature, carry out enzyme digestion reaction 2.0~

    3.0h, obtain the second enzymolysis solution, the add-on of wherein said compound protease obtains according to the cubage of Semen arachidis hypogaeae protein in the mass concentration of described hot pressed peanut meal solution and the hot pressed peanut meal measured;

    Step 4, by the described neutral protease in described the second enzymolysis solution and conjugated protein enzyme-deactivating, centrifuging and taking supernatant liquor, obtains peanut peptide solution.

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