Production method for powder containing crystalline alpha, alpha-trehalose dihydrate

Production method for powder containing crystalline alpha, alpha-trehalose dihydrate

  • CN 103,946,386 A
  • Filed: 09/12/2012
  • Published: 07/23/2014
  • Est. Priority Date: 09/21/2011
  • Status: Active Application
First Claim
Patent Images

1. contain α

  • , the preparation method of the crystal powder of α

    -trehalose dihydrate compound, described crystal powder contains the α

    more than 98.0 quality % that convert based on anhydride, α

    -trehalose, described method is containing having the following steps;

    make isoamylase separate enzyme with the alpha-glycosyl trehalose synthase that derives from genus arthrobacter microorganism with the trehalose that derives from genus arthrobacter microorganism with ring Star Dri 5-glucanotransferase and act on liquefying starch, then, glucoamylase is played a role, obtain containing α

    , the step of the liquid glucose of α

    -trehalose, from above-mentioned liquid glucose, make α

    , the step of α

    -trehalose dihydrate compound crystal structure, and, collect the α

    of crystallization by centrifugation, α

    -trehalose dihydrate compound crystal, make its slaking, dry step, described method feature is, by being used as the natural type that derives from series bacillus microorganism belonging to genus or recombinant type enzyme or their mutant enzyme of above-mentioned ring Star Dri 5-glucanotransferase, without the fractionating step that adopts column chromatography, make the α

    in above-mentioned liquid glucose, α

    -content of trehalose exceedes the 86.0 quality % that convert based on anhydride.

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