Contain α, the preparation method of the powder of alpha trehalose dihydrochloride dihydrate crystal

Contain α, the preparation method of the powder of alpha trehalose dihydrochloride dihydrate crystal

  • CN 103,946,386 B
  • Filed: 09/12/2012
  • Issued: 01/02/2018
  • Est. Priority Date: 09/21/2011
  • Status: Active Grant
First Claim
Patent Images

1. contain α

  • , the preparation method of the powder of α

    -trehalose dihydrate solvate crystal,The powder containing a, a-trehalose dihydrochloride dihydrate crystal contains more than the 99.0 mass % based on anhydride conversionα

    , α

    -trehalose,The α

    calculated based on x-ray diffractogram of powder spectrum, the crystallinity of α

    -trehalose dihydrate solvate crystal is more than 90.0%,Methods described comprises the following steps:

    Make isoamylase and ring maltodextrin-glucanotransferase and the alpha-glycosyl trehalose from Arthrobacter microorganismSynthase and from Arthrobacter microorganism trehalose separate enzyme effect in liquefying starch, then, play glucoamylaseEffect, obtain containing α

    , the step of the liquid glucose of α

    -trehalose,From above-mentioned liquid glucose, make α

    , the step of α

    -trehalose dihydrate solvate crystal crystallization, and,The α

    of crystallization, α

    -trehalose dihydrate solvate crystal are collected by centrifugation, the step for make its curing, drying,Methods described is characterized in,By using above-mentioned ring maltodextrin-glucanotransferase, without the fractionating step using column chromatography so that onThe α

    in liquid glucose is stated, α

    -content of trehalose exceedes the 86.0 mass % based on anhydride conversion, and the ring maltodextrin-Portugal gathersSugared transferaseWith the amino acid sequence shown in any one in sequence number in sequence table 1,2,3,12 or 13 orIn the range of the substrate specificity of the enzyme or enzymatic activity is not changed substantially have in sequence table sequence number 1,2nd, in the amino acid sequence shown in any one in 3,12 or 13 missing, displacement or insertion 1 with the amino acid of up to 30The amino acid sequence of residue,By controlling cooling method or intending carrying out the above-mentioned step for crystallizing a, a-trehalose dihydrochloride dihydrate crystal like control cooling methodSuddenly, whereinIt is described control cooling method refer to when using as the operating time that crystallisation step is set be set to " τ

    ", by crystallize beginning when liquidTemperature is set to " T0", the target liquid temperature at the end of crystallization is set to " Tf", when the liquid temperature in the time " t " is set into " T ", in time tThe cooling means that liquid temperature T is represented by following formula:

    T=T0-(T0-Tf)(t/τ

    )3, andThe plan refers to like control cooling method, when the liquid temperature at the time point of t=τ

    /2 is set into TmWhen, make liquid temperature T-phase for time tContinuously or by stages it is reduced to the variable quantity " T in the liquid temperature at the time point of t=τ

    /20-Tm" maintain total temperature changeMeasure " T0-Tf" more than 5% but the scope less than 50% cooling means.

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