Method of cryopreservation of tissue derived from pluripotent stem cells

Method of cryopreservation of tissue derived from pluripotent stem cells

  • CN 103,958,669 A
  • Filed: 11/22/2012
  • Published: 07/30/2014
  • Est. Priority Date: 11/25/2011
  • Status: Active Application
First Claim
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1. freezing preservation derives from the method for the tissue of multipotential stem cell, and described method comprises following (1) to (3):

  • (1) the first step, makes the tissue that derives from multipotential stem cell contact with the cytoprotective solution that contains sulfoxide and chain polyvalent alcohol,(2) second step, remains on the tissue that derives from multipotential stem cell in the described the first step described in contacting with described cytoprotective solution in Cryosreservation solution, and(3) the 3rd steps, under refrigerant exists, freezing be kept in described second step, be maintained in described Cryosreservation solution described in derive from the tissue of multipotential stem cell.

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