Fusion protein efficiently combined with PD-1 and VEGF, coding sequence and application thereof

Fusion protein efficiently combined with PD-1 and VEGF, coding sequence and application thereof

  • CN 103,965,363 B
  • Filed: 02/06/2013
  • Issued: 01/15/2021
  • Est. Priority Date: 02/06/2013
  • Status: Active Grant
First Claim
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1. An isolated fusion protein consisting of the following peptide fragments:

  • 1) an extracellular domain peptide of human PD-L2;

    2) the peptide fragment of 2 nd immunoglobulin-like structural domain of human FLT-1 extracellular region or/and the peptide fragment of 3 rd immunoglobulin-like structural domain of KDR extracellular region; and

    3) an Fc fragment of a human immunoglobulin;

    wherein;

    the amino acid sequence of the extracellular region peptide segment of the human PD-L2 is shown as SEQ ID NO;

    1 is shown in the specification;

    the amino acid sequence of the peptide fragment of the 2 nd immunoglobulin-like domain of the human FLT-1 extracellular region is shown as SEQ ID NO;

    3 is shown in the specification;

    the amino acid sequence of the peptide segment of the 3 rd immunoglobulin-like structural domain of the human KDR extracellular region is shown as SEQ ID NO;

    5 is shown in the specification;

    the Fc fragment has no ADCC effect;

    the Fc fragment is positioned on the side of the fusion protein close to the C terminal relative to the peptide fragments described in

         1) and

         2);

    the peptide fragment of the extracellular region of the human PD-L2, the peptide fragment of the 2 nd immunoglobulin-like domain of the extracellular region of the human FLT-1 and the peptide fragment of the 3 rd immunoglobulin-like domain of the extracellular region of the KDR are respectively and independently one or two copies.

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