Cellobiohydrolase variants and polynucleotides encoding same

Cellobiohydrolase variants and polynucleotides encoding same

  • CN 103,998,605 B
  • Filed: 12/20/2012
  • Issued: 08/03/2021
  • Est. Priority Date: 12/20/2011
  • Status: Active Grant
First Claim
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1. A cellobiohydrolase variant having a substitution at one of positions 112, 154, 197, 228, 256, 261, 262, 306, 322, 332, 344, or 375 of the mature polypeptide of SEQ ID No. 2, wherein the variant has cellobiohydrolase activity;

  • wherein the content of the first and second substances,which is substituted with His at a position corresponding to position 112 of the mature polypeptide of SEQ ID NO. 2;

    which is substituted with Met at a position corresponding to position 154 of the mature polypeptide of SEQ ID NO. 2;

    which is substituted with Tyr at a position corresponding to position 197 of the mature polypeptide of SEQ ID NO. 2;

    Which is substituted with Val at a position corresponding to position 228 of the mature polypeptide of SEQ ID NO. 2;

    substituted with Leu at a position corresponding to position 256 of the mature polypeptide of SEQ ID NO. 2;

    substituted with Leu at a position corresponding to position 261 of the mature polypeptide of SEQ ID NO. 2;

    which is substituted with Lys at a position corresponding to position 262 of the mature polypeptide of SEQ ID NO. 2;

    which is substituted with Ala at a position corresponding to position 306 of the mature polypeptide of SEQ ID NO. 2;

    which is substituted with Ile at a position corresponding to position 322 of the mature polypeptide of SEQ ID NO. 2;

    it is substituted with Asp at a position corresponding to position 332 of the mature polypeptide of SEQ ID NO. 2;

    which is substituted with Phe at a position corresponding to position 344 of the mature polypeptide of SEQ ID NO. 2;

    substituted with Glu at a position corresponding to position 375 of the mature polypeptide of SEQ ID NO. 2;

    the variant has at least 99%, but less than 100% sequence identity to the amino acid sequence of the parent cellobiohydrolase.

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