Protein fractionation based on pi

Protein fractionation based on pi

  • CN 104,024,856 A
  • Filed: 11/05/2012
  • Published: 09/03/2014
  • Est. Priority Date: 11/04/2011
  • Status: Active Grant
First Claim
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1. a method for separated one or more target proteins from sample, described method comprises,In chamber, add sample, described sample contains the protein mixture that comprises one or more target proteins, it is characterized in that, described chamber comprises the first and second electrodes and between the above electrode of described chamber wall and by least one proton syringe or the hydroxyl syringe of the described sample separation in Bipolar Membrane and described chamber;

  • Use described proton syringe or hydroxyl syringe in described chamber, to produce pH gradient, and cross over described electrode application voltage, thereby the protein-based isoelectric point in described protein (pI) in described chamber is located;

    At the port being communicated with passage fluid, catch one or more protein;

    AndThe described protein that one or more are caught is carried out to gel electrophoresis.

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