Method for cell count, cell counter and cell counting procedure
Method for cell count, cell counter and cell counting procedure
 CN 104,080,922 B
 Filed: 01/22/2013
 Issued: 07/21/2017
 Est. Priority Date: 01/31/2012
 Status: Active Grant
First Claim
1. a kind of method for cell count, it is the method for counting culture cell, it is characterised in thatThe image of the culture cell is obtained,The image of cell mass and the image of separate cell are separated by the image,The circle that image according to the separate cell carries out separate cell is approximate, and calculate the separate cell cell number andAverage area,Correction coefficient is calculated according to following formula (i) or formula (ii),(i) projected area >
 =1.0802 ×
10 of cell mass^{5}cm^{2}When,Correction coefficient=1.1003 ×
(projected area of cell mass, ×
10^{5}cm^{2}) 0.1885；
(ii) the projected area ＜
1.0802 ×
10 of cell mass^{5}cm^{2}When,Correction coefficient=1,According to the projected area of the cell mass, with after the spherical volume for calculating the cell mass divided by correction coefficient, so as to calculateGo out the volume of the cell mass,Average area according to the separate cell calculates the volume of the separate cell,By the volume divided by the volume of the separate cell of the cell mass, the cell number of the cell mass is calculated.
Chinese PRB Reexamination
Abstract
The present invention provides a kind of method for cell count, in cell culture, even in cell it is intensive and when forming threedimensional cell mass, also can not break up the cell mass and count culture cell number exactly.This method is as follows：The image of culture cell is obtained, and the image of cell mass and the image of separate cell are separated by the image, the image and the image of separate cell according to cell mass, calculate the cell number of cell mass and the cell number of separate cell respectively.
3 Claims

1. a kind of method for cell count, it is the method for counting culture cell, it is characterised in that
The image of the culture cell is obtained, The image of cell mass and the image of separate cell are separated by the image, The circle that image according to the separate cell carries out separate cell is approximate, and calculate the separate cell cell number andAverage area, Correction coefficient is calculated according to following formula (i) or formula (ii), (i) projected area >  =1.0802 ×
10 of cell mass^{5}cm^{2}When,Correction coefficient=1.1003 ×
(projected area of cell mass, ×
10^{5}cm^{2}) 0.1885；(ii) the projected area ＜
1.0802 ×
10 of cell mass^{5}cm^{2}When,Correction coefficient=1, According to the projected area of the cell mass, with after the spherical volume for calculating the cell mass divided by correction coefficient, so as to calculateGo out the volume of the cell mass, Average area according to the separate cell calculates the volume of the separate cell, By the volume divided by the volume of the separate cell of the cell mass, the cell number of the cell mass is calculated.
 =1.0802 ×

2. a kind of method for cell count, it is the method for counting culture cell, it is characterised in that
The volume of separate cell is preset, The image of the culture cell is obtained, The image of cell mass and the image of separate cell are separated by the image, The circle that image according to the separate cell carries out separate cell is approximate, and calculate the separate cell cell number andAverage area, Correction coefficient is calculated according to following formula (i) or formula (ii), (i) projected area >  =1.0802 ×
10 of cell mass^{5}cm^{2}When,Correction coefficient=1.1003 ×
(projected area of cell mass, ×
10^{5}cm^{2}) 0.1885；(ii) the projected area ＜
1.0802 ×
10 of cell mass^{5}cm^{2}When,Correction coefficient=1, According to the projected area of the cell mass, with after the spherical volume for calculating the cell mass divided by correction coefficient, so as to calculateGo out the volume of the cell mass, By the volume divided by the volume of the separate cell set in advance of the cell mass, the cell of the cell mass is calculatedNumber.
 =1.0802 ×

3. a kind of cell counter, it is the cell counter for counting culture cell, it is characterised in that possessed：

The unit of the image of the acquirement culture cell, By the image separate cell mass image and separate cell image unit and The circle that image according to the separate cell carries out separate cell is approximate, and calculate the separate cell cell number andAverage area, Correction coefficient is calculated according to following formula (i) or formula (ii), (i) projected area >
=1.0802 ×
10 of cell mass^{5}cm^{2}When,Correction coefficient=1.1003 ×
(projected area of cell mass, ×
10^{5}cm^{2}) 0.1885；(ii) the projected area ＜
1.0802 ×
10 of cell mass^{5}cm^{2}When,Correction coefficient=1, According to the projected area of the cell mass, with after the spherical volume for calculating the cell mass divided by correction coefficient, so as to calculateGo out the volume of the cell mass, the average area according to the separate cell calculates the volume of the separate cell, by describedThe volume of the volume of cell mass divided by the separate cell, calculates the unit of the cell number of the cell mass.

Specification(s)