Method for cell count, cell counter and cell counting procedure

Method for cell count, cell counter and cell counting procedure

  • CN 104,080,922 B
  • Filed: 01/22/2013
  • Issued: 07/21/2017
  • Est. Priority Date: 01/31/2012
  • Status: Active Grant
First Claim
Patent Images

1. a kind of method for cell count, it is the method for counting culture cell, it is characterised in thatThe image of the culture cell is obtained,The image of cell mass and the image of separate cell are separated by the image,The circle that image according to the separate cell carries out separate cell is approximate, and calculate the separate cell cell number andAverage area,Correction coefficient is calculated according to following formula (i) or formula (ii),(i) projected area >

  • =1.0802 ×

    10 of cell mass-5cm2When,Correction coefficient=1.1003 ×

    (projected area of cell mass, ×

    10-5cm2) -0.1885;

    (ii) the projected area <

    1.0802 ×

    10 of cell mass-5cm2When,Correction coefficient=1,According to the projected area of the cell mass, with after the spherical volume for calculating the cell mass divided by correction coefficient, so as to calculateGo out the volume of the cell mass,Average area according to the separate cell calculates the volume of the separate cell,By the volume divided by the volume of the separate cell of the cell mass, the cell number of the cell mass is calculated.

View all claims
    ×
    ×

    Thank you for your feedback

    ×
    ×