Preparation method of fructus momordicae extract applicable to industrial production

Preparation method of fructus momordicae extract applicable to industrial production

CN
  • CN 104,086,614 A
  • Filed: 07/25/2014
  • Published: 10/08/2014
  • Est. Priority Date: 07/25/2014
  • Status: Active Grant
First Claim
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1. a preparation method who is applicable to industrial Fructus Monordicae extract, is characterized in that:

  • comprise the following steps;

    (1) raw material pre-treatment;

    Grosvenor Momordica fresh fruit is cleaned, suitably broken, take that not smash Grosvenor Momordica fruit seed be degree;

    (2) extract;

    in the Grosvenor Momordica after fragmentation, add hydrophilic solvent, be heated to 80~

    90 ℃

    , refluxing extraction 3~

    4 times is extracted 1.0~

    1.5h at every turn, and the gained solution that will at every turn reflux merges, and filters, and obtains extracting solution;

    (3) concentrated;

    by gained extracting solution in step (2), be concentrated into 1/3~

    5/9 of original volume, be cooled to 15~

    25 ℃

    , standing rear filtration, obtains concentrated solution;

    (4) centrifugal, ultrafiltration;

    gained concentrated solution in step (3) is carried out centrifugal, carry out ultrafiltration after centrifugal, obtain filtrate;

    (5) the multistage absorption of macroporous resin is with separated;

    use >

    =3 macroporous resin chromatography columns, in the mode of series connection, gained filtrate in step (4) is carried out to upper prop absorption, upper column flow rate is 0.5~

    1.0 BV/h, standing 2~

    3h after upper prop completes;

    With the deionized water that is equivalent to total resin >

    =2 times volume, at 50~

    60 ℃

    , carry out wash-out, elution flow rate is 1.0~

    2.0 BV/h, then respectively with hydrophilic solvent right >

    =3 macroporous resin chromatography columns carry out respectively wash-out and collect respectively hydrophilic solvent elutriant, >

    =3 parts of hydrophilic solvent elutriants;

    (6) nanofiltration, concentrated;

    use respectively nanofiltration membrane to carry out nanofiltration processing a gained >

    =3 part hydrophilic solvent elutriant in step (5), till then carrying out respectively the sharp degree 25~

    30 of vacuum concentration to hundred, obtain >

    =3 portions of concentrated solutions;

    (7) dry;

    by gained in step (6) >

    =after 3 portions of concentrated solutions be dried respectively, obtain >

    =3 kinds of specifications contain the product of momordica glycoside V.

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