One plant using glucose as the colibacillus engineering of substrate synthesis muconic acid

One plant using glucose as the colibacillus engineering of substrate synthesis muconic acid

  • CN 104,099,284 B
  • Filed: 07/01/2014
  • Issued: 12/04/2018
  • Est. Priority Date: 07/01/2014
  • Status: Active Grant
First Claim
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1. one plant using glucose as the colibacillus engineering of substrate synthesis muconic acid, which is characterized in that introduce 2,3- dihydroxyYl benzoic acid and enhances metabolic pathway of the Escherichia coli itself from glucose to 2,3-DHB to the metabolic pathway of muconic acid;

  • InstituteThe metabolic pathway of 2,3-DHB to MA is stated by expressing heterologous 2,3- dihydroxy-benzoic acid decarboxylase and catechol 1, the bis- oxygenations of 2-Enzyme is realized;

    Metabolic pathway of the reinforcing Escherichia coli itself from glucose to 2,3-DHB, by expressing branch'"'"'s acid isomerEnzyme, different chorismate lyase, 2,3- dihydro -2,3- dihydroxy-benzoic acid dehydrogenase are realized, aroG gene is also expressedaroGfbrWith shikimate kinase gene, to release phenylalanine to the feedback inhibition of isodynamic enzyme AroG, and strengthen shikimic acid pathway;

    The nucleotide sequence of the gene entX of 2, the 3- dihydroxy-benzoic acid decarboxylase is encoded as shown in SEQ ID NO.1;

    Coding instituteCatechol 1 is stated, the nucleotide sequence of the gene catA of 2- dioxygenase is as shown in SEQ ID NO.2;

    Encode the chorismic acidThe nucleotide sequence of the gene entC of isomerase is as shown in SEQ ID NO.3;

    Encode the gene of the different chorismate lyaseThe nucleotide sequence of entB is as shown in SEQ ID NO.4;

    Encode the base of the 2,3- dihydro -2,3- dihydroxy-benzoic acid dehydrogenaseBecause the nucleotide sequence of entA is as shown in SEQ ID NO.5;

    The aroG gene aroGfbrNucleotide sequence such as SEQShown in ID NO.6;

    The nucleotide sequence of the gene aroL of the shikimate kinase is encoded as shown in SEQ ID NO.7;

    It is describedEntX and catA is co-expressed using pACYCDuet-1 or pETDuet-1 as carrier;

    The aroGfbrWith aroL withPCDFDuet-1 is that carrier is co-expressed, and described entC, entB, entA are co-expressed with plasmid pRSFDuet-1.

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