Method for producing natural killer cells, natural killer cells produced thereby, and composition for treating cancers and infectious diseases containing the same

Method for producing natural killer cells, natural killer cells produced thereby, and composition for treating cancers and infectious diseases containing the same

  • CN 104,204,194 A
  • Filed: 12/18/2012
  • Published: 12/10/2014
  • Est. Priority Date: 12/22/2011
  • Status: Active Grant
First Claim
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1. a method of producing NK cell, comprises the following steps:

  • (i) separate peripheral blood leucocyte and NK cell from human peripheral;

    (ii) containing static cultivation NK cell in the substratum of anti-CD 3 antibodies, cytokine and feeder cell, to stimulate by cell contact;

    (iii) after the static cultivation in completing steps (ii), by adding cytokine, anti-CD 3 antibodies and feeder cell repetitive stimulation cell, and carry out static cultivation to stimulate, cause cell contact;

    (iv) after completing static cultivation, to adding in cell containing static cultivation or the required cytokine of suspension culture and the substratum of analogue, keeping cell concn and cytokine concentration in constant level, carry out static cultivation or suspension culture.

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