Gene inactivation allowing immediate growth on xylose medium by engineered zymomonas

Gene inactivation allowing immediate growth on xylose medium by engineered zymomonas

  • CN 104,220,590 A
  • Filed: 12/19/2012
  • Published: 12/17/2014
  • Est. Priority Date: 12/20/2011
  • Status: Active Application
First Claim
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1. prepare a method for fermentation single cell bacterium (Zymomonas) cell that can utilize wood sugar, comprising:

  • A) fermentation single cell bacterium host cell is provided;

    B) in described cell, create genetic modification at least one endogenous gene, described endogenous gene codes has under NADPH exists the aldose reductase of the EC1.1.1.21 by xylose being the ability of Xylitol;

    AndC) in described cell, xylose utilization pathways metabolism is introduced;

    Wherein the order of step (b) and (c) is not specified, and the wherein said aldose reductase activity utilizing the Zymomonas cells of wood sugar to have with lack step (b) genetic modification Zymomonas cells compared with decrease beyond 90%.

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