Preparation method for formamide phenazine biological fungicide

Preparation method for formamide phenazine biological fungicide

  • CN 104,222,098 A
  • Filed: 06/08/2013
  • Published: 12/24/2014
  • Est. Priority Date: 06/08/2013
  • Status: Active Application
First Claim
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1. a preparation method for formamide phenol piperazine biological bactericide, is characterized in that:

  • comprise the following steps, and following material is all represent with mass fraction;

    (1) get the cfcc 10466 Pseudomonas chlororaphis bacterial strain trophosome of the deposit number of vegetative stage, be placed in wavelength in 280-320 nanometer, ultraviolet lamps irradiates 12-18h, obtain mutant strain;

    (2) 2 parts of analysis for soybean powder, 0.2 part of dusty yeast and 50-60 part running water are mixed, then be heated to 90-100 DEG C, then filter, in the filter residue obtained, add 3 parts of running water again, continue to filter 1-2 time;

    (3) in the filtrate after filtration, 0.3-0.4 part glucose, 0.25-0.35 part peptone, 0.04-0.07 part potassium dihydrogen phosphate, 0.02-0.04 part magnesium sulfate is added, stirring and dissolving, add 1.2-1.8 part corn starch and 5.3 parts of running water more wherein, be mixed to get solution, solution pH value is regulated to be 7.5-7.8 with the sodium hydroxide solution that molar concentration is 5-7mol/L, obtain pre-culture solution, pre-culture solution is distributed in triangular flask, be stoppered bottle stopper, triangular flask is heated to 120-125 DEG C, sterilizing 20-40min, obtains culture fluid;

    (4) control temperature is 28-30 DEG C, mixing speed is 180-200rpm, the mutant strain obtained in step (1) is carried out in culture fluid a ring inclined-plane lawn inoculation, incubation time is 30-40h, obtains the bacterial strain containing formamide phenol piperazine one pack system;

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